前蛋白转化酶枯草溶菌素9(PCSK9)重组蛋白说明书
Recombinant Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9)
[PROPERTIES]
Source: Prokaryotic expression
Host: E.coli
Residues: Gln454~Cys679
Tags: N-terminal His Tag
Subcellular Location: Secreted, Lysosome, Golgi apparatus, Endoplasmic reticulum lumen
Purity: > 97%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 0.1% SKL, 5% Trehalose.
Original Concentration: 80µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 7.3
Predicted Molecular Mass: 25.4kDa
Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[USAGE]
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[SEQUENCE]
[IDENTIFICATION]
[IMPORTANT NOTE]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用