前列腺蛋白类脂肪酶B(LIPB)重组蛋白说明书

Recombinant Lipophilin B, Prostatein Like (LIPB) 

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Glu22~Val90

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Skeletal Muscle, Thymus. Subcellular Location: Secreted. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM

EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 37.6kDa

Accurate Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are asfollows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
 2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of0.1-1.0

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date underappropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用