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上海信裕生物科技有限公司
NELFB抗体

NELFB抗体

商家询价

产品名称： NELFB抗体

英文名称： NELFBAntibody

产品编号： XY16418-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 NELFB抗体Applications

In-house applications ELISA, IP, IF, WB     Application not tested? Please see our Guarantee.
Species specificity Human,Mouse,Rat; other species not tested.
Images
Prev1NextView more validation data
  • 16418-1-AP;BxPC-3 cellsBxPC-3 cells were subjected to SDS PAGE followed by western blot with 16418-1-AP( NELF-B Antibody) at dilution of 1:1000
  • 16418-1-AP;mouse brain tissueIP Result of anti-NELF-B (IP:16418-1-AP, 3ug; Detection:16418-1-AP 1:500) with mouse brain tissue lysate 6000ug.
  • 16418-1-AP;BxPC-3 cellsImmunofluorescent analysis of BxPC-3 cells using 16418-1-AP( NELF-B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in BxPC-3 cells, HepG2 cells
Positive IP detected in Mouse brain tissue
Positive IF detected in BxPC-3 cells
Recommended dilution WB : 1:500-1:5000
IP : 1:200-1:2000
IF : 1:10-1:100
NELFB抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen InformationNELFB抗体
Immunogen NELFB fusion protein ag9599 Full name Cofactor of BRCA1
Calculated molecular
weight		 580aa,66 kDa;475aa,54 kDa Observed molecular 
weight		 66 kDa
GenBank accession 
number		 BC011892 Gene ID (NCBI) 25920
Gene symbol COBRA1 Synonyms COBRA1, cofactor of BRCA1, DKFZp586B0519, KIAA1182, Negative elongation factor B, NELF B, NELFB
Background

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.NELFB抗体

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