促销时间：2016年3月15-2016年6月14日

促销内容：原价是4122 促销价格是3500

Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应

美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。

ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21
种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。
确保了实验结果的重复性和连贯性。

请登录Sciencell公司官方网站（www.zqxzbio.com 或www.sciencellonline.com ）以确保购买正规公司产品

人脐静脉内皮细胞说明：
货号：8000


血管内皮细胞对维持血管动态平衡起着重要作用。它们合成、分泌凝血和纤溶系统的激活因子和抑制因子、影响血
小板粘附和聚集的调节因子。内皮细胞还释放控制细胞增殖和调节血管壁紧张度的分子。这些过程都可以用培养的
细胞进行体外研究，人脐静脉和动脉内皮细胞是这类研究中最常用的细胞类型。除了普通的内皮细胞特征例如
“鹅卵石状”，VIII因子阳性着色，能摄取乙酰基化低密度脂蛋白，人脐血管内皮细CD31染色阳性。用IL-1或TNF-α
预先处理过的细胞也能选择性地表达E-selectin和VCAM。 人脐静脉内皮细胞(HUVEC)提取于人脐带静脉组织，
原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证，
经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。

推荐培养基:(ECM, Cat. No. 1001)

The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize and
secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition
to mediators that influence the adhesion and aggregation of blood platelets. Endothelial cells also release
molecules that control cell proliferation and modulate vessel wall tone. Many of the endothelial processes
can be studied in vitro using cultured cells, and human umbilical vein endothelial cells (HUVEC) are the
most commonly used cell type for such studies. Except for the common endothelial cell features such as
"cobblestone" morphology, positive staining for Factors VIII and the ability to take up acetylated low-density
lipoprotein [1], HUVEC stein positively with CD-31 [2]. Cells pretreated with IL-1 or TNF-alpha also selectively
express E-selectin [3] and VCAM [4].
HUVEC from ScienCell Research Laboratories are isolated from human umbilical vein. HUVEC are
cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HUVEC
are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM)
and by uptake of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and
fungi. HUVEC are guaranteed to further expand for 15 population doublings at the conditions provided by
ScienCell Research Laboratories.

Reference
1. Morgan, D. M. L. (1996) Isolation and culture of human umbilical vein endothelial cells. In "Human Cell
Culture Protocols" (Gareth E. Jones, eds.) Humana Press, Totowa, New Jersey, pp.104-109.
2. Newman, P. J. et al., (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin
gene superfamily. Science 247: 1219-1222.
3. Bevilaqua, M. P. et al., (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils
related to complement regulatory proteins and lectins. Science 243:1`160-1165.
4. Osborn, L. et al., (1989) Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial
protein that binds to lymphocytes. Cell 59:1203-1211.

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